目的 探讨神经生长因子(nerve growth factor, NGF)在变应性鼻炎发病中的作用。方法 建立SD大鼠变应性鼻炎的模型，利用酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)法测定实验性变应性鼻炎鼻粘膜组织中的神经生长因子的含量。结果 实验性变应性鼻炎鼻粘膜的神经生长因子含量(-x±s,下同; n=9)为(6.65±0.42) ng/g湿重，对照组(n=6)为(3.51±0.29)ng/g湿重，二者相比差异有显著性(P<0.001)。结论 神经生长因子可能通过调节神经肽的表达和释放和/或者通过活化免疫细胞(如肥大细胞)而在变应性鼻炎的发生中起重要作用。

目的：探讨TrKA和p75神经营养因子受体在嗅神经母细胞瘤的表达及其临床意义。方法：应用免疫组化和双荧光标记法对10例鼻腔嗅神经母细胞瘤及5例正常鼻腔嗅黏膜及5例鼻咽癌组织进行TrKA和p75神经营养因子受体检测。结果：免疫组化检测发现10例鼻腔嗅神经母细胞瘤TrKA和p75蛋白均呈阳性表达，5例正常鼻腔嗅黏膜及5例鼻咽癌组织均为阴性表达；双荧光标记显示所有肿瘤组织中TrKA和p75蛋白均在同一细胞表达。结论：TrKA和p75神经营养因子受体的过度表达可能与嗅神经母细胞瘤的发生和发展有关；TrKA和p75神经营养因子受体检测可作为嗅神经母细胞瘤的一种辅助诊断方法。

The unclear DNA content was measured with flow cytometry (FCM) in 167 specimens of normal nasopharyngeal epithelium (NE), adjacent tissue to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). All 20 patients with NE showed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 pa-tients with ATC and 2 (33%) of 6 patients with NE, positive to EBVCA-IgA test, showed nondiploid. In addition, cellular proliferation index (PI) of diploid NPC and diploid ATC, though differred nonsignificantly, was significantly increased as compared with that of NE (P<0.01). The rate of cervical metastasis in nondiploid NPC was significantly higher than that in diploid NPC (P<0.025).

Cellular morphology and nuclear DNA content were meas-ured with point-counting technique and flow cytometry (FCM) respectively in normal nasopharyngeai epithelium (NE), tissue adjacent to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). The results showed that all 20 patients with NE show-ed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 pa-tients with ATC and 2 (33%) of 6 patients with NE positive to EBVCA-IgA test showed nondiploid. A total of 19 morphometric parameters of the cell were measured and calcu-lated. The results showed that with malignant transformation of NE, most of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal NE and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasia changed significantly as com-pared with those normal NE, simple hyperplasia or mctaplasia and NPC groups. Cytomorphometric analysis seems to give strong support to the concept that dysplasia is among the transitional stages of disease process between normal NE and NPC. Our results show that morphometric analysis and FCM may offer valuable and objective criteria in the early diagnosis of NPC and premalignant disease.