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2005年05月17日

【期刊论文】Exposure of the shaded side of apple fruit to full sun leads to up-regulation of both the xanthophyll cycle and the ascorbate-glutathione cycle

马锋旺, Fengwang Ma, Lailiang Cheng*

Plant Science 166(2004)1479-1486,-0001,():

-1年11月30日

摘要

Approximately 80 days after full bloom, well-exposed fruit on the south part of the canopy of mature Liberty/M9 apple (Malus domestica Borkh.) trees were randomly assigned to one of the following two treatments. Some fruits were turned about 180◦ to expose the original shaded side to full sun, whereas the rest served as untreated controls. On days 0, 1, 2, 4, 7 and 10 after treatment, fruit peel samples were taken from the original shaded side of the treated fruit and both the sun-exposed side and the shaded side of the control fruit at midday, to determine photosynthetic pigments, enzymatic and non-enzymatic antioxidants. Maximum photosystem II (PSII) efficiency, measured as Fv/Fm (maximum fluorescence) of chlorophyll fluorescence at pre-dawn, was higher in the shaded side than the sun-exposed side of the control fruit. Fv/Fm of the original shaded side decreased sharply after 1 day exposure to full sun, and then gradually recovered to a similar value of the sun-exposed side of the control fruit by day 10. The shaded side of the control fruit had much lower xanthophyll cycle pool size, conversion and antioxidant enzymes and also soluble antioxidants of the ascorbate–glutathione cycle than the sun-exposed side. Xanthophyll cycle pool size of the original shaded side increased in response to full sun exposure, reaching a similar value of the sun-exposed side by day 10. Almost all the xanthophyll cycle pool was converted to zeaxanthin and antheraxanthin starting from the first day of exposure to full sun. In contrast, chlorophyll content decreased whereas lutein or neoxanthin content remained unchanged. In response to full sun exposure, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase and total pool size and reduction state of both ascorbate and glutathione, all increased to the corresponding values found in the sun-exposed side of the control fruit over a 10-day period. It is concluded that both xanthophyll cycle and the ascorbate-glutathione cycle in the original shaded side are up-regulated in response to full sun exposure to minimize photo-oxidative damage and contributes to its acclimation to full sun.

Apple fruit, Ascorbate-glutathione cycle, Malus domestica, Photoprotection, Acclimation, Xanthophyll cycle

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2005年05月17日

【期刊论文】高温胁迫对猕猴桃幼苗叶片某些生理效应的影响*

马锋旺, 耶兴元, 王顺才, 梁东

西北农林科技大学学报(自然科学版),2004,32(12):33~37,-0001,():

-1年11月30日

摘要

在45℃下对猕猴桃一年生盆栽苗进行2,4,6,8,10h的高温胁迫,测定高温胁迫下叶绿素含量、相对膜透性、丙二醛(MDA)含量、抗氧化酶活性和抗氧化剂含量及热激蛋白等生理生化指标。结果表明,高温胁迫下叶绿素含量下降,相对膜透性增大,MDA 含量增加; 超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸还原酶(APX)活性均在胁迫初期呈上升趋势,随处理时间延长,活性下降;随胁迫时间延长,还原型谷胱甘肽(GSH) 含量先升后降,抗坏血酸(ASA)含量总体呈下降趋势,谷胱甘肽还原酶(GR)和脯氨酸含量一直呈上升趋势;热激蛋白(HSP)在高温下被诱导。

高温胁迫, 猕猴桃, 幼苗叶片, 生理效应

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2005年05月17日

【期刊论文】梨树花芽休眠解除与活性氧代谢的关系

马锋旺, 邵浩, 马锋旺*

植物生理与分子生物学学报,2004,30(6):660-664,-0001,():

-1年11月30日

摘要

梨树(Pyrus bretschneideri Regd.)自然休眠和休眠解除时,花芽的活性氧代谢发生变化。O2-·产生速率和H2O2的含量在休眠期间上升,在休眠后期下降。抗氧化系统中SOD活性在自然休眠期呈下降趋势,自然休眠结束活性上升。POD和CAT活性在自然休眠期上升。抗氧化物质ASA和GSH的含量随休眠进行而下降,休眠解除过程中重新升高。APX和GR的活性在休眠期间活性下降,休眠结束活性迅速上升。这些结果表明:花芽的休眠与活性氧的代谢有很大关系。

梨树, 花芽, 休眠, 活性氧代谢

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2005年05月17日

【期刊论文】梨叶片离体培养和植株再生

马锋旺, 徐凌飞, 王之, 任小林, 曹晓雁

园艺学报,2002,29(4):367~368,-0001,():

-1年11月30日

摘要

用梨(Pyrus)4个栽培品种的叶片诱导不定芽。暗培养20d,取试管苗顶部幼嫩叶片,远轴面向下接触培养基,叶片再生效率高。八月红品种的叶片再生效率高于其它3个品种。在NN69+TDZ 1.0mg/L+IBA 0.5mg/L 培养基上,八月红梨叶片再生频率为100%,平均再生芽数为5.78。八月红梨和水晶梨叶片再生苗在附加IBA的1/2MS培养基上生根。

梨, 叶片培养, 植株再生

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2005年05月17日

【期刊论文】杏花芽分化后期碳素同化物的分配

马锋旺, 李利红, , 白静, 李玲

果树学报,2004,21(1):73~75,-0001,():

-1年11月30日

摘要

以雌蕊败育率不同的2个杏品种Tyinthos(败育率低)和金杏(败育率高)为试材,于花芽分化后期(9月中旬)各选一枝条进行14G标记,研究其14G同化物的分配规律。结果表明,Tyinthos花芽在各个时期的同化物比强、花芽的同化物分配率、叶片的光合产物利用率均高于金杏。

杏, 碳素同化物, 花芽分化

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