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冯庆玲, Q. L. Feng, , J. Wu, G. Q. Chen, F. Z. Cui, T. N. Kim, J. O. Kim
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-1年11月30日
To investigate the mechanism of inhibition of silver ions on microorganisms, two strains of bacteria, namely Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus), were treated with AgNO3 and studied using combined electron microscopy and X-ray microanalysis. Similar morphological changes occurred in both E. coli and S. aureus cells after Ag+ treatment. The cytoplasm membrane detached from the cell wall. A remarkable electron-light region appeared in the center of the cells, which contained condensed deoxyribonucleic acid (DNA) molecules. There are many small electron-dense granules either surrounding the cell wall or depositing inside the cells. The existence of elements of silver and sulfur in the electron-dense granules and cytoplasm detected by X-ray microanalysis suggested the antibacterial mechanism of silver: DNA lost its replication ability and the protein became inactivated after Ag+ treatment. The slighter morphological changes of S. aureus compared with E. coli recommended a defense system of S. aureus against the inhibitory effects of Ag+ ions.
silver ions, antibacterial mechanism, DNA molecule, morphological changes, transmission electron microscopy
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【期刊论文】Crystal orientation, toughening mechanisms and a mimic of nacre
冯庆玲, Q.L. Feng*, F.Z. Cui, G. Pu, R.Z. Wang, H.D. Li
Materials Science and Engineering C 11 2000 19-25,-0001,():
-1年11月30日
Based on the investigations of crystal structure of nacre using SEM, TEM and XRD, it is proposed that there exists a domain structure of crystal orientation in the nacre. The orientation domain consists of continuous 3-10 tablets along the direction perpendicular to nacreous plane, and 1-5 tablets in a single lamina. The tablets in a domain are crystallographic identical in three dimensions. From the crack morphologies, it is found that the crack deflection, fibre pull-out and organic matrix bridging are the three main toughening mechanisms acting on nacre. The organic matrix plays an important role in the toughening of this biological composite. The biomimetically synthesized composite made of alumina and kevlar showed significant increase in the fracture energy compared with the single ceramics. The soluble proteins extracted from nacre can induce aragonite and the one from prism can induce calcite grown with the preferred orientation of 104. The insoluble proteins control the nucleation site and thus lead to a finer crystallization of CaCO3.
Nacre, Crystal structure, Toughening mechanisms, Biomimetics, Biomineralization
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【期刊论文】A simple biomimetic method for calcium phosphate coating
冯庆玲, F. Li a, Q.L. Feng a*, F.Z. Cui a, H.D. Li a, H. Schubert b
Surface and Coatings Technology 154 (2002) 88–93,-0001,():
-1年11月30日
A biomimetic calcium phosphate coating is expected to enhance the bioactivity and bone inductivity of human implants. This study presents a very simple, highly effective biomimetic method to obtain a calcium phosphate coating on a titanium substrate. Using NaH2PO4, a stable solution was prepared with high calcium and phosphate ion concentrations. This solution turned to a supersaturated calcification solution (SCS) when NaHCO3 was added. The addition of NaHCO3 elevated the pH value of the solution gradually and steadily. A uniform coating approximately 40 mm thick was found on the substrates after 24 h immersion. The compositions of the coatings were adjustable from hydroxyapatite (HA) to HAydicalcium phosphate dihydrate (DCPD). The calcium phosphate deposits were investigated by means of scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transformed infrared (FT-IR) spectroscopy.
Calcium phosphate coating, Biomimetics, Titanium
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【期刊论文】Controlled crystal growth of calcium phosphate on titanium surface by NaOH-treatment
冯庆玲, Q.L. Feng a, *, H. Wang a, F.Z. Cuia, T.N. Kim b
Journal of Crystal Growth 200 (1999) 550-557,-0001,():
-1年11月30日
A simple supersaturated calcification solution (SCS) was used to deposit calcium phosphate (Ca-P) on the surface of NaOH-treated titanium (NaOH-Ti), in order to study the corresponding deposition mechanism of calcium phosphate (Ca-P). No Ca-P coating from SCS was found on titanium without NaOH-treatment, while NaOH-Ti possessed the capability to induce a Ca-P coating on the titanium surface. Octacalcium phosphate (OCP) crystals were first grown on an NaOH-Ti surface, followed by hydroxyapatite (HA) with a[001] preferential orientation on OCP. It is found that two factors controlled the growth of Ca-P crystals on NaOH-Ti from SCS. First, the surface morphology of NaOH-Ti characterized with crevices seems to be beneficial for inducing a Ca-P coating from SCS; second, the basic hydroxyl, Ti-OH, radical has increased in NaOH-Ti with the increase of treating time and concentration, which facilitate the nucleation of Ca-P crystals.
Calcium phosphate, Deposition mechanism, NaOH-treatment, Titanium
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冯庆玲, Q.L. Feng a, *, G. Pu a, Y. Pei a, F.Z. Cui a, H.D. Li a, T.N. Kim b
Journal of Crystal Growth 216 (2000) 459-465,-0001,():
-1年11月30日
The function of biomacromolecules during biomineralization was studied by simulating the nucleation and growth of calcium carbonates in vitro. The synthetic crystals nucleated and grew on different organic (EDTA-insoluble proteins) and inorganic (silicon) matrices with EDTA-soluble proteins extracted from mollusk shells. The polymorph and morphology of the crystals were investigated using scanning electron microscope (SEM), X-ray diffraction (XRD) and transmission electron microscope (TEM) with selected area electron diffraction (SAED). It is found that different proteins have specialties to induce different crystals with special morphologies. The soluble proteins extracted from nacre can induce aragonite and the one from prism can induce calcite grown with a preferred orientation of[104]. The crystals induced by the same soluble proteins have the same polymorph and morphology. The insoluble proteins insoluence the density of nucleation sites as well as the sizes and quantities of the crystals.
Aragonite, Biomacromolecules, Biomineralization, Calcite
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