Purpose: Hypericin, a specific inhibitor of protein ki-base C, has been reported to have potential as a thera-peutic dru for proliferative vitreoretinopathy (PVR) in vitro and in vivo. In the present strdies, we analyzed the dynamic changesin Ca2+ in flux and freeintracellular Ca2+ concentration Ca2+ of cultured human retinal pigment epith (RPE) cells after stimulation with hypericin in an attempt to elucidate its mechnism as a therapeutic drug for PVR, Methods: RPE cells were plated in a special plastic dish and then stimulated with 100 nM phorbol-12myristate-13-acetate (PMA) and/of 6 hypericin con-centrations (0.5, 1, 2, 3, 4 and 5 μM), after which Ca2+ influx and Ca2+ were determined using the fluores-cence Ca2+ dye fluo-3 AM and laser scanning confocal microscopy. Results: The fluorescence in resting Rpe Cells was strong and distributed throughout the cells. The nucleus appeared more fluorescent than the cyto-plasm. After stimulation with 0.5μM hypoericin, no ob-vious change of Ca2+ influx and Ca2+ was observed. In contrast, stimulation with higher concentrations of hy-pericin (1-5μM) led to a rapid decrease in Ca2+influx and Ca2+ which was significantly different from those de-tected without hypericin (contro experiments). In addl-tion , no significant differences in Ca2+ were found between 1 and 5μM hypericin used. Stimulation with hypericin, which was applled immediately after preincu-bation with PMA for 24 h not further change Ca2+ influx and Ca2+ Conclusion: In Rpe cells, high concen-trations of hypericin (1-5μM) significantiy inhibit Ca2+ influx and induce a decrease in Ca2+. Therefore, hypericin has potential as a therapeutic drug for PVR maybethrough its inhibition of the Ca2+ influx pathway.

由于青光眼的早期诊断、发病机制、致病基因筛选及功能测定、视神经损伤与保护、滤过术后瘢痕调控、干细胞培养与自然动物模型形成、流行病学研究等基本问题尚处于探讨阶段，因此有必要回顾我国近5年来青光眼的临床与基础研究进展情况，分析青光眼研究领域存在的问题，明确今后青光眼的研究方向，进一步推动青光眼的临床与基础研究深入发展。

目的：探讨体外诱导小鼠胚胎干细胞（ESCs）分化为神经干细胞（NSCs）的可能性。方法：ESCs经胚胎体阶段，以视黄酸及视网膜müller细胞诱导，在NSCs选择性培养基中筛选培养扩增7d，观察形态变化，采用RT-PCR法检测nestin、glutaminase、Brn-3基因表达及免疫细胞化学法检测nestin等NSCs特异性标志物，并对其扩增及分化能力进行观察。结果：ESCs经初级诱导，NSCs选择性培养基筛选培养7d后，形成大量神经球样结构，可扩增传代。绝大部分神经球样结构呈nestin抗原阳性，整合BrdU，表达nestin、glutaminase、Brn-3基因，且可分化为神经元及神经胶质样细胞。结论：视黄酸及müller细胞诱导的ESCs，经选择性培养基筛选培养可获得NSCs，有望为青光眼等神经变性疾病提供新的治疗途径。

目的探讨治疗原发性闭角型青光眼三种手术方式的适应症和初步临床疗效观察。方法采用非随机临床对照研究方法。拟订手术适应症，对临床收治的63例72眼原发性闭角型青光眼进行手术处理：单纯抗青光眼手术——复合式小梁切除术（Trabeculectomy,Trab）、青-白联合手术——复合式小梁切除联合超声乳化白内障吸除+晶体囊袋折叠式人工晶体植入术（Phacotrabeculectomy+IOL，PhacoTrab+IOL）和单纯白内障手术——超声乳化白内障吸除＋晶体囊袋折叠式人工晶体植入术（Phaco+IOL）。比较不同适应症下三种手术方式初步的临床疗效。包括眼压控制情况、前房深度和房水流畅系数（C值）的变化，超声生物显微镜（UBM）观察房角和滤过泡，以及用抗青光药物辅助情况及并发症等。随访时间平均（11.7±4.9）个月。结果均经统计学处理。结果三组术后在随访期内均能将眼压控制在理想范围，术后眼压较术前明显降低（三组P值均＜0.001），前房深度：PhacoTrab+IOL和Phaco+IOL组术后明显较术前加深，差异有统计学意义（P值均＜0.001），但Trab组术前后改变不明显（P＞0.05）；UBM观察房角：PhacoTrab+IOL和Phaco+IOL组明显较术前加宽或重新开放，相应组C值术后较术前提高，差异有统计学意义（P分别＜0.001和＜0.01），Trab组房角变化不明显，但术后C值较术前亦有改善（P＜0.001）；三组均未见严重术中并发症；术后早期炎症反应最轻者为Phaco+IOL组；远期并发症Trab组有5眼发生低眼压。最后对视力的变化也作了讨论。结论不同手术方式适合不同的病人情况。超声乳化白内障吸除术因其具有相对房角开放和C值改善以及视力提高等优势，可考虑作为原发性闭角型青光眼治疗的选择性手段之一，但要注意适应症的选择。

目的：建立人眼小梁细胞体外滤膜培养体系及通透阻力测定的模型。方法：将永生化的人眼小梁细胞株用酶消化法传代于细胞培养池的PET膜上，观察细胞的生长情况，同时分别在细胞融合后3、5d和1、2周，应用内皮细胞电阻测量仪（EVOM）测定滤膜上单层小梁细胞电阻，客观评价其通透阻力。结果：人眼小梁细胞在滤膜上生长良好，单层融合后3、5d和1、2周的平均电阻分别为（36.4±1.4）Ω、（35.0±1.7）Ω、（36.1±2.9）Ω、（39.3±3.0）Ω，各时间点无显著统计学差异（P=0.305）。结论：人眼小梁细胞滤膜上培养单层融合后，在不同时间段其阻力基本稳定。滤膜培养体系及应用EVOM对其通透阻力的测定可作为今后对小梁细胞特性研究的一个新的较好模型。