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【期刊论文】Kinetic model of lysozyme renaturation with the molecular chaperone GroEL
董晓燕, Xiao-Yan Dong, Yu-Bing Wang, Xiao-Guang Liu & Yan Sun, *
Biotechnology Letters 23: 1165-1169, 2001.,-0001,():
-1年11月30日
From the renaturation kinetics of denatured/reduced lysozyme assisted by the molecular chaperone GroEL, a simplified kinetic model was established based on the competition between protein folding and aggregation. In the presence of GroEL and ATP, the aggregate formation was a second order reaction. With 2mM ATP, a renaturation yield of 90% at a high renaturation rate was obtained when the molar ratio of GroEL to lysozyme was 1: 1.
GroEL,, kinetics,, lysozyme,, model,, renaturation
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董晓燕, Yang Liu, Xiao-Yan Dong, Yan Sun*
Journal of Colloid and Interface Science 290(2005)259-266,-0001,():
-1年11月30日
The nonionic surfactant of sorbitan trioleate (Span 85) was modified with Cibacron Blue F-3GA (CB) as an affinity surfactant (CB-Span 85) to form affinity-based reversed micelles in n-hexane. The reversed micelles formed by the mixture of Span 85 and CB-Span 85 conjugate were extensively characterized in water content, hydrodynamic radius, and aggregation number. The results show that the water content and hydrodynamic radius of the reversed micelles were significantly increased by the introduction of CB ligands (CB-Span 85 conjugate), and the reversed micelles with CB-Span 85 conjugate had a wider aggregation number distribution than the Span 85 reversed micelles. Using lysozyme as a model protein, protein solubilization by the reversed micelles was investigated. Lysozyme solubilization increased significantly with the coupled CB concentration, indicating that the extraction was based upon the affinity interactions between lysozyme molecules and the CB ligand. High solubilization of lysozyme was obtained by the affinity-based reversed micelles of 62.7mmol/L Span 85 with coupled CB higher than 0.25mmol/L. Lysozyme recovery was carried out using a stripping solution of high ionic strength. The recovered lysozyme exhibited an activity equivalent to the native lysozyme and its secondary structure was also unchanged. The results indicate that the reversed micellar system would find potential application in protein separation.
Affinity-based reversed micelles, Span 85, Cibacron blue F-3GA, Water content, Hydrodynamic radius, Solubilization, Lysozyme
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【期刊论文】Lysozyme refolding with immobilized GroEL column chromatography
董晓燕, Xiao-Yan Dong, Hui Yang, Yan Sun*
J. Chromatogr. A 878(2000)197-204,-0001,():
-1年11月30日
A refolding chromatography with immobilized molecular chaperonin GroEL was studied for the reactivation of denatured-reduced lysozyme. The effect of denaturant concentration (guanidine hydrochloride, 0.1-1.5M) in the elution buffer, the elution flow-rate, and the loading concentration and volume of the substrate protein on the reactivation yield was studied. All the operating parameters showed minor effects on the recovery yield of lysozyme mass, which remained at 90-100%, but exhibited relatively notable influences on the specific activity of the recovered lysozyme. For example, thereexisted an optimum denaturant concentration of about 1 M at which the highest yield of specific activity (up to 97%) was obtained. Using the immobilized GroEL column, 3ml of the lysozyme (1mg/ml) per batch could be refolded at an overall yield of 81%, which corresponded to a refolding productivity of 54mg per l gel per h. At comparable reactivation yields (over 80%), this value of productivity was over four-times larger as that of the size-exclusion refolding chromatography reported previously (12mg per 1gel perh), indicating the advantage of the present system for producing a high throughput in protein refolding operations.
Immobilized chaperonins, Lysozyme, Proteins
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