Based on the continuous wavelet transform (CWT), three types frequency spectra, wavelet frequency spectrum (WFS), point frequency spectrum (PFS) and time frequency spectrum (TFS), were developed. Two data sets were simulated and treated with the proposed spectra, the results indicated that WFS could extract the frequency information, which was like Fourier analysis but more accurate, PFS could obtain the frequency at any moment, TFS could show frequency change with time. These abilities of PFS and TFS were impossible for Fourier analysis. An oscillating chemical signal was processed with WFS and TFS. From the processed results, two points could be learned about the oscillating chemical reaction: one was the oscillating chemical reaction was a mixture one including two or more complex kinetics processes, the velocity of the switch from the reduced state (RS) to the oxidized state (OS) was faster than the reverse switch (from OS to RS); the other was increase of KBrO3 could decrease the velocities of both switches, which led to the oscillating period became longer.

To estimate the number of peaks and to find the individual peak positions in an overlapped signal, a new method called maximum spectrum of continuous wavelet transform (MSCWT) was developed by extracting the maximum coefficients of continuous wavelet transform (CWT). The peak position in MSCWT was the same as that in its original signal. In this process, CWT was performed not on a single dilation but on an appreciation dilation range. To obtain such a range, a new criterion was introduced to choose a center dilation, which was used to form the dilation range. If Cdilation denoted the center dilation, the proper dilation range was [Cdilation-6 (2, Cdilation+1

An electron transfer reaction between ascorbic acid (H2A) in an aqueous solution and oxidizing agent in an organic solution immiscible with water has been studied by thin-layer cyclic voltammetry (TLCV) for charge transfer at the interface between two immiscible electrolyte solutions (ITIES). As an antioxidant, H2A provide electrons through the aqueous/organic interface to reduce Fc

The interaction of the two new synthesized transition-metal complexes, ML2 (M=Co, Cu, L= 1, 8-dihydroxyethyl-1, 3, 8, 10, 13-hexa-azacyclotetradecane) with calf thymus DNA was probed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Adding deoxyribonucleicacid (DNA) into [CoL]2+ and [CuL]2+ solution, the ip value of all the peaks of [CoL]2+ and [CuL]2+ significantly decreased in proportion to concentration of DNA. Glassy carbon electrodes (GCEs) were modified with DNA by adsorption, and it was electrochemically characterized with transition-metal complexes, [ML]2+. The DNA modification layer on the GCE is unstable to alkali and to heat, but stable to acid solutions and very stable in long stock in a dry state. It could be seen that peak potential shifted positively and the peak current increased significantly. The electrochemical parameters, binding constant (kn+) and binding sites(s) were calculated by a nonlinear regression method.

The electrochemical characteristics of kanamycin onto self-assembled monolayer (SAM) modified gold electrode (SAM/Au) is investigated by cyclic voltammetry. In the potential range 0-0.6 V, Cu (II) yields a pair of stable redox waves at the bare gold electrode. Epa is located at 0.189 V and Epc at 0.254 V. In contrast, Cu (II) is reduced at a more positive potential and a decreasing current at the kanamycin SAM/Au electrode. Cu (II) and kanamycin can form a stoichiometry complex with chemical ratio of 2:1. The interaction of Cu(II)-kanamycin complex with calf thymus DNA is also studied in the solution. And the interactive mode between Cu (II)-kanamycin complex and DNA is verified by the fluorescence method. Binding constants (K) of the Cu (II)-kanamycin complex to DNA and binding site size (s) are calculated from voltammetric data and equal to 1.5×10-7 l/mol and 4 bp, respectively.