本文选择低磷耐性较强的羽扇豆和低磷耐性较弱的番茄两种作物，在不同磷水平下水培，对其根系分泌特性进行了比较研究。结果表明，在低磷条件下，羽扇豆酸性磷酸酶和有机酸分泌能力比番茄高2-3倍。番茄体内磷的生理临界浓度显著比羽扇豆为高，可能是其低磷耐性较低的重要原因。

Phyt1 gene was successfully transferred into soybean (Glycine max L. Merr. c.v. Jilin35, China) by Agrobacterium mediated method in this study. The results of molecular detections (Southern, Nouthern and Western) demonstrated that the phyt1 transgene was expressing in the transgenic soybean plant To and T1 generations, thereby caused a higher phytase activity and a lower phytate content in the ransgenic plants. This study is the first report about the transgenic soybean plants containing lower phytate.

1.4-kb phytase gene amplified by RT-PCR from A. ficuum total RNA was successfully cloned into the expression vector pYES2, subsequently the recombinant expression vector pYPA1 was obtained. By the transformation with pYPA1, a transgenic S. cerevisiae that could express extracellular functional phytase was constructed. The successful construction of this engineering yeast strain made the roundwork for research on feed additive and microbial fertilizer.

The cDNA encoding quail (coturnix) ovalbumin was cloned from quail oviduct by the method of RT-PCR, which was inserted into the P. pastoris genome downstream of the methanol inducible 5' alcohol oxidase (AOX) promoter to replace the AOX1 gene using the plasmid vector pPIC9, and a recombinant P. pastoris strain efficiently secreting quail ovalbumin was successfully constructed. ELISA analysis using a polyclonal antibody raised against quail ovalbumin showed that, induction by 0.75% methanol for 48h led to synthesis of secreted quail ovalbumin to give around 5.45 g l-1, respectively. The recombinant ovalbumin was purified into homogeneity later through ion exchange and gel filtration chromatography. SDS-PAGE analysis revealed that, compared to natural ovalbumin, the recombinant ovalbumin is glycosylated in the similar extent by P. pastoris.

黑芥子酶是一类催化水解芥子油苷的同工酶，Pyk10是在拟南芥根和下胚轴中特异表达的黑芥子酶基因，Pyk10基因的启动子具有根组织特异性。根据已发表的序列，用PCR方法从拟南芥基因组中扩增并克隆了Pyk10基因启动子片段。序列分表明：该片段全长1454bp，与已发表序列同源性达到98%。该启动子片段中含有多种在其他植物启动子中发现的通用启动子元件，并含有器官和组织特异性转录因子的结合位点有ACGT-、CANNTG-、GATA-以及I盒等顺式元件。Pyk10根特异性启动子的成功克隆，为培育抗根部病虫害和营养高效利用型转基因植物奠定了基础。