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【期刊论文】A high efficient method for filling-in the cohesive ends of double-stranded DNA
李明刚, Shao-Hui Yang, Xin Li, Weidong Li, Jianhua Hou, and Ming-Gang Li*
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-1年11月30日
This paper reports a high efficient method for making cohesive end of double-stranded DNA into blunt end. Klenow fragment and Pfu DNA polymerase were used to fill-in the cohesive ends, and their illing-in efficiency and the corresponding cloning efficiency were compared. The results indicated the filling-in efficiency of Pfu DNA polymerase is 1.89 times that of Klenow fragment, and the difference was significant (p﹤0.01).
Klenow fragment, Pfu DNA polymerase, Filling-in
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