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李明刚, Minggang Li, Mitsuru Osaki, Idupulapati Madhusudana Rao and Toshiaki Tadano
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-1年11月30日
Phosphorus (P) deficiency increased the secretion of phytases from roots of various plant species. The secretory phytases were collected with a dialysis membrane tube for 24 hours from roots of sixteen plant species grown with low or adequate supply of P in nutrient solutions. The activity of not only secretory phytase, but also acid phosphatase, increased with the low P treatment in all of the plant species examined. Secretion of phytase by the roots under P-deficient conditions was highest in Brachiaria decumbens CIAT 606, Stylosanthes guianensis CIAT 148 and tomato, moderate in Brachiaria brizantha CIAT6780, Stylosanthes guianensis CIAT 2950, Alfalfa, white clover and orchard grass, and lowest in Andropgon gayanus CIAT 621, Stylosanthes capitata CIAT 10280, upland rice, timothy, redtop, alsike clover, red clover and white lupin plants. SDS-PAGE and immunoblotting analysis showed that the phytases with molecular weight of 35-40 kD could be detected in seven of the sixteen plant species tested. These results indicate that the secretory phytase may provide an efficient mechanism for certain plants to utilize inositol hexaphosphate in soil.
anti-phytase IgG, phosphorus deficiency, phytic acid, secretory acid phosphatase, secretory phytase.,
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