高效聚乙烯醇降解菌的选育及应用
首发时间:2023-04-04
摘要:印染废水中聚乙烯醇(polyvinyl alcohol ,PVA)降解慢、生物处理效率低。本论文从印染废水活性污泥中筛选并经诱变获得一株36 h完全降解1g L-1 PVA的菌株Sphingopyxis sp. H3,降解所需时间比诱变前缩短了45.5%。诱变前后PVA降解酶序列未发生变化,活性未提高。细胞膜完整性增加,通透性减弱,抗逆性增强。显微分析及电镜观察发现突变菌株胞外黏性物质减弱、分散度显著增加。qRT-PCR显示诱变后胞外多糖合成分泌能力减弱,磷酸戊糖途径及TCA循环加强,这些都与其对PVA降解能力的提升有关。Sphingopyxis sp. H3对含有PVA的印染废水处理系统的抗PVA冲击力具有良好的提升效果,且有助于活性污泥系统PVA降解能力的恢复。
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Breeding and application of high efficient polyvinyl alcohol degrading bacteria
Abstract:The degradation of polyvinyl alcohol (PVA) in printing and dyeing wastewater is slow and the biological treatment efficiency is low. In this study, the strain Sphingopyxis sp. H3 was obtained from activated sludge of printing and dyeing wastewater after screening and mutagenesis, which completely degraded 1 g L-1 PVA in 36 h. The degradation time was reduced by 45.5% after mutagenesis. The sequences of PVA degrading enzymes were not changed and their activities were not improved. Membrane integrity increases, permeability decreases and stress resistance increases.Microanalysis and electron microscopy showed that the extracellular viscosity of the mutant strain was weakened and the dispersion increased significantly. qRT-PCR showed that after mutagenesis, extracellular Breeding and mechanism analysis of highly efficient polyvinyl alcohol degrading bacteriapolysaccharide synthesis and secretion ability were weakened, pentose phosphate pathway and TCA cycle were strengthened, which were related to the improvement of PVA degradation ability. Sphingopyxis sp. H3 increased the ability against PVA shock loading and promoted the PVA degradation ability of activated sludge system.
Keywords: polyvinyl alcohol Sphingopyxis sp. biodegradation printing and dyeing wastewater
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