Anti-prostatitis effect of the total flavonoids of Pteris multifida based on intestinal flora interaction

• 1、College of Pharmatieutical Sciences, Soochow University, Suzhou 215123
• 2、Xi’an Jiaotong-Liverpool University, Suzhou 215000, China
• 3、The Second Affiliated Hospital of Soochow University, Suzhou 215004

Abstract：Objective: To investigate the metabolisms and materialbasis for anti-chronic prostatitis (CP) effect of flavonoids from Pteris multifida.(PME).Methods: SD rats were injected with complete Freund\'s adjuvant to establish the CP rat model, and were randomly divided into 5 groups: (1) normal control group; (2) model group; (3) positive drug prostat group; (4) PME1 group; (5) PME2 group. The prostat, PME1, and PME2 groups were given 100 mg/kg by gavage daily, while the control group and model group were given normal saline by gavage. The drug was given for 5 days and stopped for 2 days, for a total of 6 weeks.After the end of the administration, feces, blood, colon contents, and prostate tissues were collected from the rats. The colonic intestinal flora of rats in each group was analyzed by 16S rDNA sequencing. The composition and intestinal metabolism changes of PME were analyzed by HPLC and UHPLC-TOF/MS methods. Results: PME exihibited potential in alleviating inflammation and swelling in CP rats, and reduce the level of TNF-α, IL-8, and IL-10. PME could modulatinf the structure of intestinal microbiotaandretify the imbalance of intestinal microbiotabydown-regulating harmful bacteria such as Bilophilia and Dialister, and up-regulating beneficial bacteria like Lactobacillus and Ligilactobacillus. The main components of PME were apigenin, luteolin, and related flavonoid glycosides. In vivo and in vitro results showed that these flavonoid glycosides were metabolized into corresponding glycosides by intestinal flora.Conclusion: The interaction between PME and the gut microbiota may contribute to the therapeutic effects of CP by modulating the composition and metabolites of intestinal. Results: PME demonstrated potential in Relieving inflammation and swelling of CP rats, as well as reducing the expression of TNF-α, IL-8, and IL-10. Additionally, it exhibited the ability to modulate the structure and balance of intestinal flora by down-regulating harmful bacteria such as choleophilus and Dialister while up-regulating beneficial bacteria like Lactobacillus and Ligilactobacillus. The primary constituents of PME consist of apigenin, luteolin, and their glycosides. In vivo and in vitro metabolism studies revealed that intestinal flora metabolized the O-glycosides of apigenin and luteolin into their respective aglycones; however, C-glycosides remained unmetabolized and were directly absorbed into the bloodstream. Conclusion: PME can significantly improve the symptoms of CP rats, and its flavonoid glycosides can be metabolized by intestinal flora and play a role in regulating intestinal flora. The interaction between PME and intestinal flora can correct the imbalance of intestinal flora, inhibit the expression of inflammatory factors, and contribute to the treatment of CP.

Keywords： Pteris multifida. chronic prostatitis flavonoids intestinal flora