介孔聚多巴胺纳米粒修饰及药物负载研究
首发时间:2024-04-22
摘要:目的:合成介孔聚多巴胺纳米粒,负载化疗药物吡柔比星,经透明质酸修饰后,制备得到MPDA-THP@PDA-HA NPs,并进行表征。方法:利用一锅法在碱性条件下合成MPDA NPs,在弱碱性条件下负载THP,并将PDA和HA修饰MPDA表面,得到MPDA-THP@PDA- HA NPs。通过SEM和TEM观察纳米粒形貌特征,采用马尔文粒度仪检测纳米粒的尺寸并进行Zeta电位测定、用HPLC检测包封率、载药量和药物体外释放、进行红外光谱、光热转换等实验,对其性质进一步考察。结果:MPDA具有球形和介孔结构,粒径397 nm,Zeta电位表明纳米粒体系稳定。负载药物的MPDA-THP@PDA-HA NPs包封率为18.3%,载药量为13%,该纳米粒具有良好的pH响应性释药和明显的光热升温能力。结论:成功合成并修饰载药MPDA NPs。其稳定性良好,粒径均一,具有较好的载药能力和光热转换能力。为肿瘤的化学/光热协同治疗提供一种有效的治疗策略。
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Modification and drug loading of mesoporous polydopamine nanoparticles
Abstract:Objective: To synthesize mesoporous polydopamine nanoparticles loaded with chemotherapeutic drug pirarubicin and modified with hyaluronic acid to prepare MPDA-THP@PDA- HA NPs and characterize them.Methods: MPDA NPs was synthesized by one-pot method under alkaline condition, loaded with THP under weakly alkaline condition, and the MPDA surface was modified with PDA and HA to obtain MPDA-THP@PDA-HA NPs.The morphology characteristics of the nanoparticles were observed by SEM and TEM, the size of the nanoparticles was detected by Malvern particle size analyzer and Zeta potential was determined, the encapsulation rate, drug load and drug release in vitro were detected by HPLC, and the properties were further investigated by infrared spectroscopy and photothermal conversion experiments.Results: The MPDA has spherical and mesoporous structure, the particle size is 397 nm, and the Zeta potential indicates that the nanoparticle system is stable.The encapsulation rate of MPDA-THP@PDA-HA NPs loaded with drugs was 18.3%, and the drug loading was 13%. The nanoparticles had good pH response to drug release and obvious photothermal heating ability.Conclusion: MPDA NPs was synthesized and modified successfully.It has good stability, uniform particle size, good drug loading ability and photothermal conversion ability.To provide an effective treatment strategy for chemical/photothermal synergistic therapy of tumor.
Keywords: Drug delivery Mesoporous polydopamine nanoparticles Pirarubicin Hyaluronic acid
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