溶菌酶-利奈唑胺自组装纳米粒的构建及其抗生物膜感染的研究
首发时间:2024-04-24
摘要:目的:以溶菌酶为原料,制备负载利奈唑胺的抗生素纳米粒,用于治疗生物膜感染。方法:通过反溶剂沉淀法制备溶菌酶-利奈唑胺自组装纳米粒(Ly-LZD NPs),透射电镜观察纳米粒形貌,Zeta电位分析仪检测粒径和电位,使用红外光谱分析法分析纳米粒特征官能团,使用高效液相色谱法检测载药量。通过结晶紫染色实验,测试了该纳米粒对耐甲氧西林金黄色葡萄球菌(MRSA)生物膜的形成和破坏作用。结果:Ly-LZD NPs平均粒径为92.26 ± 0.85 nm,PDI为0.121,透射电镜下观察呈现均匀球形且表面光滑,载药量为45.23% ± 0.905。生物膜实验结果显示,相对于裸药利奈唑胺,Ly-LZD NPs显示出更强的抑制生物膜形成能力,并且对已形成的生物膜有一定的破坏作用。生物相容性实验显示,Ly-LZD NPs对细胞的活性不会产生影响,溶血率约1%,可忽略不计。结论:通过自组装制备Ly-LZD NPs,提高了利奈唑胺破坏MRSA生物膜的能力,并有效抑制生物膜形成,同时显示出良好的生物相容性,这种纳米粒可用于治疗由生物膜引发的顽固性感染。
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Construction of lysozyme-Linezolid antibiotic nanoparticles and their resistance to biofilm infection
Abstract:Construction of lysozyme-Linezolid antibiotic nanoparticles and their resistance to biofilm infectionct: Objective: To prepare antibiotic nanoparticles loaded with linezolid using lysozyme as raw material for the treatment of biofilm infection. Methods: Lysozyme -Linezolid self-assembled nanoparticles (Ly-LZD NPs) were prepared by reverse-solvent precipitation method. The morphology of the nanoparticles was observed by transmission electron microscopy, the particle size and potential were detected by Zeta potential analyzer, the characteristic functional groups of the nanoparticles were analyzed by infrared spectroscopy, and the drug loading was detected by high performance liquid chromatography. The effect of the nanoparticles on the formation and destruction of methicillin-resistant Staphylococcus aureus (MRSA) biofilm was tested by crystal violet staining. Results: The average particle size of Ly-LZD NPs was 92.26 ± 0.85nm, PDI was 0.121, the surface was smooth and uniform under transmission electron microscopy. The drug loading was 45.23% ± 0.905. The results of biofilm experiment showed that compared with linezolid, Ly-LZD NPs showed a stronger ability to inhibit biofilm formation, and had a certain destructive effect on the formed biofilm. The biocompatibility test showed that Ly-LZD NPs had no effect on the activity of the cells, and the hemolysis rate was about 1%, which was negligible. Conclusion: The self-assembly preparation of Ly-LZD NPs improves the ability of linezolid to destroy MRSA biofilm, effectively inhibits biofilm formation, and shows good biocompatibility. This nanoparticle can be used to treat refractory infections caused by biofilm.
Keywords: self-assembled nanoparticles Biofilm Lysozyme Linezolid
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溶菌酶-利奈唑胺自组装纳米粒的构建及其抗生物膜感染的研究
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