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程克棣

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期刊论文

Cloning and Sequencing of Hydroxylase Genes Involved in Taxol Biosynthesis

程克棣Jun Tu Ping Zhu* Ke-di Cheng* and Chao Meng

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摘要/描述

Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxuschinensis mainly by the single specific-primer PCR (SSP-PCR) method. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol biosynthesis. These findings imply that the two new genes are closely related to the biosynthesis of taxol/taxoids. Data Accession No: AF545833 and AY374652.

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