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期刊论文
Comparison of high-speed counter-current chromatography instruments for the separation of the extracts of the seeds of Oroxylum indicum
Journal of Chroonmatography A, 1063(2005)241-245,-0001,():
Analytical Milli high-speed counter-current chromatography (HSCCC) was used for the selection and optimization of the two-phase solvent system to separate flavonoids from the extracts of the seeds of Oroxylum indicum. The optimum solvent system obtained from Milli-CCC was also the best solvent system for preparative HSCCC and led to the successful separation of two crude flavonoids from the seeds of O. indicum by Lab/Prep (laboratory preparative) HSCCC using different sized coils. Four flavonoids were isolated by preparative HSCCC: baicalein-7-O-diglucoside (25.0mg, 92% purity), baicalein-7-o-glucoside (50.4mg; 95% purity), baicalein (75mg; purity 98%) and chrysin (100 mg; purity 98%).
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