A series of thermoresponsive gating membranes, with a wide range of grafting yields, were prepared by grafting poly (N-isopropylacrylamide) (PNIPAM) onto porous poly (vinylidene fluoride) (PVDF) membrane substrates with a plasma-induced pore-filling polymerization method. The effect of grafting yield on the gating characteristics of thermoresponsive gating membranes was investigated systematically. The results showed that the grafting yield heavily affected both the water flux responsiveness coefficient and the thermoresponsivity of the membrane pore size. When the grafting yield was smaller than 2.81%, both the flux responsiveness coefficient and the thermoresponsivity of the membrane pore size increased with an increase in the grafting yield; however, when the grafting yield was higher than 6.38%, both the flux responsiveness coefficient and the thermoresponsivity of the membrane pore size were always equal to 1; i. e., no gating characteristics existed anymore. Diffusional permeation experiments showed that two distinct types of temperature responses were observed, depending on the grafting yield. The diffusional coefficient of a solute across membranes with low grafting yields increased with temperature, while that across membranes with high grafting yields decreased with temperature. To get a desired or satisfactory thermoresponsive gating performance, the membranes should be designed and prepared with a proper grafting yield.