Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography，成果详细信息-中国科技论文在线

邓泽元

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Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography

邓泽元， Li Li a ， Rong Tsao b ， * ， Zhiqiang Liu a ， Shuying Liu a ， Raymond Yang b ， J. Christopher Young b ， Honghui Zhu b ， Zeyuan Deng c ， Mingyong Xie c ， Zhihong Fu c

Journal of Chromatography A, 1063(2005)161-169，-0001，（）：

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Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165mg of acteoside and 17.5mg of isoacteoside from 978mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.

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