Strain LBA9402Bin19 of Agrobacterium rhizogens, harboring binary vector of Bin19, incited roots in vitro on hypocotyl segments of cabbage, oilseed rape and cauliflower. Among these roots, the transformed roots containing Kn gene were resistant to kanamycin and grew on the medium eontaining 50mg/L kanamyein, whereas untransformed roots were not resistant to kanamycin and abandoned. Among three genotypes of Brassica crops tested, cauliflower had the highest root induction percentage while cabbage produced the highest root transformation frequency. Within the population of 50 transgenic lines, The introduction of Kn' gene from binary vector Bin19 was not always synchronized with that of ags and mas genes from Ri T-DNA.