Two saponins were isolated from the roots of Ardisia mamillata HANCE. Their structures were established on the basis of MALDI-TOFMS, 1H, 13C NMR and 2D NMR (COSY, HOHAHA, HETCOR, HMBC and ROESY) spectra, and on chemical evidence, to be ardisimamilloside A, 3-O-(a-L-rhamnopyranosyl-(14 2)-b-D-glucopyranosyl-(144)-[b-D-glucopyranosyl-(142)]-a-L-arabinopyranosyl)-3b, 16a, 28a-trihydroxy-13b, 28-epoxy-oleanan-30-al; and ardisimamilloside B, 3-O-(a-L-rhamnopyranosyl-(142)-b-D-glucopyranosyl-(144)-[b-D-glucopyranosyl-(142)]-a-L-arabinopyranosyl)-3b-hydroxy-13b, 28-epoxy-oleanan-16-oxo-30-al. 7 2000 Elsevier Science Ltd. All rights reserved.