In order to compare the adjuvant activity of compound Chinese herbal medicinal ingredients (cCHMIs) and filtrate best prescription anddose, two initial screened cCHMIs were prepared and mixed with Newcastle disease (ND) vaccine respectively at three doses to vaccinate17-day-old chickens, taking the non-adjuvant and oil adjuvant vaccines as controls. On day 7, 14, 21, 28, 35 and 42 after vaccination, thedynamic changes of peripheral lymphocyte proliferation and serum antibody titers in the chickens were determined by means of MTT methodand hemagglutination inhibition test. The results showed that two cCHMIs could promote peripheral lymphocyte proliferation and enhanceserum antibody titer, of which adjuvant activity was similar to and even stronger than oil adjuvant in promoting lymphocyte proliferation atsome time points, and there was a certain dose-effect relationship. The best adjuvant activity of cCHMIs 2 in promoting cellular immunityoccurred at middle dose, and cCHMIs 1 in enhancing humoral immunity, at high dose. Based on good synergistic effects of their components,two cCHMIs would be expected as new-type immunologic adjuvants to substitute for oil adjuvant.