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期刊论文
Study on Escherichia coli alkaline phosphatase conformation by phosphorimetry in the presence of denaturant
H. -R. Zhang et al. Spectrochimica Acta Part A 59 (2003) 3185-3191，-0001，（）：
The influence of different denaturants on the phosphorescence spectrum and lifetime decay of Escherichia coli alkaline phosphatase (AP) was investigated. Phosphorescence intensity and lifetime of tryptophan residue (Trp-109) decrease upon addition of guanidine hydrochloride, ethylene diamine tetraacetic acid, and urea or decreasing acidity. The experiments show that AP undergoes different pathways with different denaturants and that the activation energy data, DS8" and DH8" further confirm that there is a stable intermediate state between the folded and unfolded AP states in solution.
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