Hydrophobic charge induction chromatography (HCIC) has been proven to be an efficient technique for antibodypurification. Several HCIC adsorbents were prepared with macroporous cellulose-tungsten carbide compositebeads (Cell-TuC) as the matrix. First, the cellulose beads were activated by allyl bromide (AB) or divinylsulfone (DVS), and then they were coupled with three types of mercaptoheterocyclic groupss4-mercaptoethyl-pyridine hydrochloride (MEP), 2-mercapto-1-methyl-imidazole (MMI), and 2-mercapto-benzimidazole(MBI)sas the HCIC ligands. Four types of HCIC adsorbents were obtained, labeled Cell-TuC-AB-MEP,Cell-TuC-DVS-MEP, Cell-TuC-DVS-MMI, and Cell-TuC-DVS-MBI. The activation and coupling conditionswere optimized for high ligand density. The isotherm adsorption of immunoglobulin of egg yolk (IgY) onfour HCIC adsorbents were investigated. High adsorption capacities of IgY could be obtained for all fouradsorbents at pH 7, and low adsorption of IgY at pH 4 and of bovine serum albumin (BSA) at pH 7 wasobserved, which indicates that the HCIC adsorbents prepared have a potential application for antibodypurification.