A total of 985 bacterial isolates were obtained from rice leaves and other grasses. The isolates were cultared in nutrient broth (NB) medium. After Centrifugated, 8000r/min for 10min, the supernatants of each bacterial NB cultures were tested for their suppressive effects on the germination and appressorium formation of Magnaporthe grisea. Of the tested isolates, 13 isolates showed suppressive effects. Three isolates identified as Psendomonas fluorescens (Xh216), Bacillus sp. (Xh222) and Pseudomonas. Sp. (Xh240), suppressed the disease severities on the detached rice leaves with the disease severity reduction 72.3%, 70.5% and 67.4%, respectively.