Eleven high effective monoclonal antibodies to Magnaporthe grisea were screened out from 230 hybrid cell strains showing positive reaction in ELISA tests, among which monoclonal antibody 2B4 showed more highly banding to cell wall surfaces of conidia, germ tubes, appressoria, germ tubes, appressoria or/and second infective hypha by indirect-immunofluorcscence test. Ultrastructural analysis and the antigen localisition of monocloual antibody 2B4 with immuno-gold labeling found this antigen protein exists extensively on cell wails of the diftbrrent life stages above, and is a secretion protein. 6 positive cDNAs, MP1, MP2, MP3, MP4, MP5 and MP6 were screened out with 2B4 from conidium cDNA expression library. A 31kDa protein expressed from a positive clone MP1 in a prokaryotic in vitro translation system was consistent with a 15kda protein from the extractive of the fungal surfaces, and its gene cloning and functional analysis are undertaking.