The Giemsa C-banding analysis of 11 accessions of T. tauschii from the middle reaches of the Yellow River and Xinjiang province in China and common wheat Chinese Spring was made. The clear C-band polymorphic variation among the T. tauschii accessions from the middle reaches of the Yellow River and the accessions from Xinjiang was observed, which is consistent with evidence of RFLPs and esterase analysis in Chinese T. tauschii. Modification in the C-banding pattern was noticed on some D-genome chromosomes of Chinese Spring compared with T. tauschii. The C-banding polymorphic variation of secondary constriction region in chromosome arm 5DS of T. tauschii was found.