Chromosomal locations of crossability genes in tetraploid wheat Ailanmai with high crossability native to Jianyang of Sichuan province, China, was determined by aneuploid analysis employing the D-genome chromosome substitution lines of Langdon durum wheat and monosomic lines of common wheat, Abbondanza. High crossability of Ailanmai was attributed to recessive crossability alleles on chromosomes 1A, 6A and 7A. The effects of chromosomes 7A and 1A were stronger than chromosome 6A. These results indicated that high crossability in Chinese tetraploid wheat cv. Ailanmai and Chinese hexaploid wheat is controlled by different genetic systems.