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期刊论文
Hepatitis C Virus Replication in Stably Transfected HepG2 Cells Promotes Hepatocellular Growth and Tumorigenesis
JOURNAL OF CELLULAR PHYSIOLOGY 201: 447-458 (2004)，-0001，（）：
HepG2 cells stably transfected with a full-length, infectious hepatitis C virus (HCV) cDNA demonstrated consistent replication of HCV for more than 3 years. Intracellular minus strand HCV RNA was present. Minus strand synthesis was NS5B dependent, and was sensitive to interferon alpha (IFNa) treatment. NS5B and HCV core protein were detectable. HCV stimulated HepG2 cell growth and survival in culture, in soft agar, and accelerated tumor growth in SCID mice. These mice becameHCVRNApositive in blood, where the virus was also sensitive to IFNa. The RNA banded at the density of HCV, and was resistant to RNase prior to extraction. Hence,HCVstably replicates inHepG2cells, stimulates epatocellular growth and tumorigenesis, and is susceptible to IFNa both in vitro and in vivo. J. Cell. Physiol. 201: 447-458, 2004.
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