The jet boiling heat transfer of a bar water-CuO particle suspensions (nanofluids) jet impingement on a large flat surface was experimentally investigated. The experimental results were compared with those from water. The quantificational effects of the nanoparticles concentration and the flow conditions on the nucleate boiling heat transfer and the critical heat flux (CHF) were investigated. The experimental data showed that the jet boiling heat transfer for the water-CuO nanofluid is significantly different from those for water. The nanofluids have poor nucleate boiling heat transfer compared with the base fluid due to that a very thin nanoparticle sorption layer was formed on the heated surface. The CHF for the nanofluid increased compared with that of water. The reasons were that the solid-liquid contact angle decreased due to a very thin sorption layer on the heated surface and the jet and agitating effect of the nanoparticles on the subfilm layer enhance supply of liquid to the surface.