Lake Taihu, China’s third largest freshwater lake, exemplifies the severity of eutrophication problems in rapidly developing regions.We used long term land use, water quality, and hydrologic data from 26 in-lake and 32 tributary locations to describe the spatiotemporal patterns in nutrient loads, nutrient concentration, algal biomass, measured as chlorophyll a (Chl-a), in Lake Taihu. Point and nonpoint sources, as determined by chemical oxygen demand,contributed pproximately 75 and 25% of the total nutrient loads to the lake, respectively. Spatial patterns in total phosphorus (TP) and total nitrogen (TN) concentrations in Lake Taihu strongly corresponded with observed loads from adjoining rivers with high concentrations proximate to densely populated areas. Chl-a concentrations exhibited spatial patterns similar to TP and TN concentrations. Generally, nutrient and Chl-a concentrations were highest in the northwestern region of the lake and lowest in the southeastern region of the lake. Seasonally, the largest nutrient loads occurred during summer. The annual net retention rate of TP and TN in Lake Taihu was approximately 30% of the total load. This study identifies regions of the lake and the watershed that are producing more nutrients to develop targeted management strategies. Reducing external P and N input from both point and nonpoint sources is obviously critical to address water quality issues in the lake. In addition, atmospheric deposition and resuspension of existing lake sediments also likely play a role in eutrophication processes and harmful algal blooms occurrence.