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期刊论文
Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film
Nucleic Acids Research, 2004, Vol. 32, No.4 e45,-0001,():
We developed a new technique to immobilize a set of molecular beacons on an agarose film-coated slide and found that it has the ability to identify a single nucleotide difference in label-free DNA targets. The annealing properties, specificity and hybridization dynamics of the present technique were compared with those of the conventional technique that directly immobilizes molecular beacons on a planar glass slide. It is demonstrated that the molecular beacon array on an agarose film has high quench efficiency, an excellent discrimination ratio for single nucleotide mismatches and a short detection time. We hypothesize that such a low fluorescence background and high specificity molecular beacon array will find practical application in label-free, high-throughput mutation analysis and disease diagnosis.
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