In order to make use of B. penetrans the preparation of these bacteria was prepared successfully. Each gram of the specimen contains more than 3.1×10 8 spores according to determination by bloodcount plate. The method of its production are: First, infect the larvae of root-knot nematodes with the spores of B. penetrans. Second, inoculate infectable plants with the infected larvae. Third, beat the root-systems of inoculated plants after adding some water. The flowed liquid from a bronze sieve was mixed with the clear water, filtered, precipitated, air-dried under 5～10℃, and grinded into fine powder, When these powder was added into the water which contained the larvae of root-knot nematodes, the spores of B. penetrans adhered to the cuticles of these larvae in 24h. The pot experiments of tomato, chilli and cockscomb showed that the galls, egg-sacs, females, males and all-stages of larvae produced by the plant inoculated with the larvae infected with B. penetrans are about 75%～80% less than that without these bacteria. Thus it can be seen that the spores of B. penetrans can depress the populations of root-knot nematodes obviously. Therefore, it is a promising preparation for biological control.