A new method for understanding the relations between the molecular design and topological chirality has been developed on the basis of the Seifert construction of knot theory. Tis interesting problem of topological chirality has been solved by applying the topological symmetry concept to the molecular mobius ladders with citber 2n+1 or 2n half-twists (n≥1). Our results show that the T2n+1-Mobius ladders possess the point symmetry group C2n+1 when Lγ=(2n+1)m. and that the Tπ-Mobius ladders possess the point symmetry group Cπ when Lγ=2nm or Lγ=2nm+n(m=1,2,…). Hence both sets are topological chiral.