A new method for understanding the relations between molecular design and topological features has been developed on the basis of the Seifert construction in knot theory. Our result shows that the T2-molecular doubled knots possess the point symmetry group C2 and that the T2k-molecular doubled knots possess the point symmetry group C1 Hence both sets are topological chiral. When the rungs are cut down, topological symmetries of the molecular knots are unchanged, Our results led us to infer that the point symmetry group S1 is necessary and sufficient for the molecular topological rubber glove.