HEPATOCYTE transplantation (HTx) has been proposed as a potential therapeutic modality for various liver disorders by providing metabolic support during acute and chronic liver failure and replacing specific liver functions in inherited metabolic liver diseases. However, following allogenic hepatocyte transplantation into the spleens, the transplanted cells are rejected within a few days without immunosuppression. 1 We have previously demonstrated that this is related to the expression of Fas ligand (FasL) on splenocytes, which probably mediates apoptosis of the transplanted hepatocytes by Fas and FasL interaction. 2 FLIM58 is a blocking peptide for Fas ligand to interact with its receptor, FasR (CD95), and thus inhibits apoptotic cell death mediated by such interaction. 3 The present study was designed to investigate the effects of FLIM58 on the survival of allogenic intrasplenically transplanted hepatocytes.