Arsenic (As)-contaminated soil and water vary with pH and concentrations of As and P. This study examined the effects and interactions of three factors, pH, As and P, on As hyperaccumulator Pteris vittata L. to optimize plant growth and maximize As removal from contaminated sites, especially water. Two sets of hydroponic experiments were conducted using three-factor, five-level central composite design. Five levels of pH (4.5/8.0), As (0/668 mM), and P (0/1000 mM) were used to understand their individual as well as interactive effects. Plant biomass and uptake of P and As were impacted by all the three factors. Phosphorus inhibited As uptake at all concentrations, whereas As below 334 mM benefited plant growth and P uptake. Enhanced plant biomass was most likely a result of increased P uptake. Low pH enhanced plant uptake of As (pH5/5.21) and P (pH5/6.25). The fern had a relatively high biomass and P uptake at low pH/low As or high pH/high As. The referencing saddle points (turning points) were pH 6.33 and As 359 mM for plant biomass and pH 5.87 and As 331 mM for P uptake based on the response surface plot. The results suggested that optimum plant growth could be achieved by adjusting pH corresponding to As levels in the growth media, and maximum plant As hyperaccumulation by maintaining minimum P concentrations with medium pH5/5.21. Our results should be useful for developing strategies to remediate As-contaminated water using Chinese Brake fern. Published by Elsevier B.V.