In this paper, some new forms of the Cheeger’s inequalities are established for general (maybe unbounded) symmetric forms (Theorem 1.1 and Theorem 1.2), the resulting estimates improve and extend the ones obtained by Lawler and Sokal (1988) for bounded jump processes. Furthermore, some existence criteria for spectral gap of general symmetric forms or general reversible Markov processes are presented (Theorem 1.4 and Theorem 3.1), based on the Cheeger’s inequalities and a relationship between the spectral gap and the first Dirichlet and Neumann eigenvalues on local region.