Candida glycerinogenes, an osmotolerant yeast isolated from a natural sample in an environment of high osmotic pressure, had a modest sugar-tolerance and an extremely high glycerol productivity. The optimum conditions for glycerol formation by C. glycerinogenes were a temperature of 29-33℃ and a pH of 4-6. The optimum medium for glycerol production consisted of 230-250g glucose/l, 2g urea/l and 5ml corn steep liquor/l (55-65mg phosphates/l); the pH was not adjusted. The highest yield of glycerol was 64.5% (w/w) based on consumed glucose from 240g glucose/l, and the highest concentration of glycerol was 137g/l from 260g glucose/l. These results were obtained by using a 30-l agitated fermentor under optimal fermentation conditions. In ten batch-fermentations carried out in a 50,000-l airlift fermentor, an average yield of glycerol of 50.67% (w/w) and an average glycerol concentration of 121.9g/l were obtained from an average 240.6g glucose/l.