To construct an expression vector containing the E1 glycoprotein gene of rubella virus for the study on the effect of mutation of the E1 gene glyeoprotein and the analysis of phylogenetic differences of sequences,the gene encoding the E1 envelope glcoprotein was amlified from rubella virus, Jinan strain JR23, by RT-PCR and ligated into PMD-18T vector. The clones that camied the E1 gene were identified after amp selection and analysis of restrition enzyme digenstion. After sequencing this gene was analyzed by Danstar and Winstar prograns, and the map of phyolgenetic tree was dramw. The colone of E1 glycoprotein was thus constructed. It was found that the sequence differeces between JR23 strain and the TCRB strain from Japan and those between JR23 strain and Thomas strain of England were rather small with difference values of 0.9% and 1.2% respectively. Yet those between JR23 strain and BRD2 strain from Beijing and those between JR23 strain and XC379 strain from Hong Kong were comperatively larger with difference values of 7.6% and 7.3% resoectivesly. The sequece of JR23 strain with other strains was less than 3% except the NC strain (3.7%). It concludes that the construction of E1 glcoprotein gene offers an approach to study the relationship between structures and functions of E1 gene and its gene products. In the phlogenetic tree, it shows that there are significant differences in the sequences of rebella vinus isolated in China, and this might be helpful to develop an effective vaccine.