The structural transition and compaction of human telomeric G-quadruplex induced by excluded volume effect under cation-deficient conditions Jun Zhou a,b, Chunying Wei a,1, Guoqing Jia a,b, Xiuli Wang a,b, Qian Tang c, Zhaochi Feng a, Can Li a,，成果详细信息-中国科技论文在线

魏春英

60浏览
0点赞
0收藏
0分享
43下载
0评论
引用

期刊论文

The structural transition and compaction of human telomeric G-quadruplex induced by excluded volume effect under cation-deficient conditions Jun Zhou a,b, Chunying Wei a,1, Guoqing Jia a,b, Xiuli Wang a,b, Qian Tang c, Zhaochi Feng a, Can Li a,

魏春英， a State Key Laboratory of Catalysis ， Dalian Institute of Chemical Physics ， Chinese Academy of Sciences ， Dalian ， China b Graduate School of Chinese Academy of Sciences ， Beijing ， China c Bioengineering College ， Dalian University ， China

Biophysical Chemistry 136(2008)124-127，-0001，（）：

URL:

摘要/描述

The structure polymorphism of human telomeric G-quadruplex (ht-quadruplex) is currently an important topic but remains controversy. Here, we present study of the ht-quadruplex under the cation-deficient but molecular crowding conditions by circular dichroism (CD), microchip electrophoresis (MCE) and UV-melting experiments. Our results show that with concentration increasing of poly(ethylene glycol) (PEG), the structural transition of ht-quadruplex occurs accompanied by structural compaction and enhanced stabilization, which may be caused by excluded volume effect. This work also demonstrates that htquadruplex can be well assembled without cations and the structure of ht-quadruplex is actually very complex in vivo.

关键词: Human telomeric G-quadruplex ， Molecular crowding ， Excluded volume effect ， Structural transition ， Compaction

问答

暂无问题，成为第一个提问者

我要提问全部问题

【免责声明】以下全部内容由[魏春英]上传于[2011年05月13日 13时45分16秒]，版权归原创者所有。本文仅代表作者本人观点，与本网站无关。本网站对文中陈述、观点判断保持中立，不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考，并请自行承担全部责任。

我要评论

全部评论 共 0 条

本学者其他成果

同领域成果