Raman spectra of a polythiophene film measured over a temperature range 298-98K were analyzed using generalized two-dimensional correlation spectroscopy. In the 1600-1250cm-1 region, the asynchronicity of the bands indicates the existence of different species in the investigated polythiophene sample. At least four distinct bands at 1485, 1454, 1418, and 1360cm-1 were identified. Among them, the band at 1454cm-1, attributed to the neutral species of PTh, is found to be sensitive to the temperature-induced structural changes of the polythiophene film, while the bands of the oxidized species are less sensitive to the temperature change during the cooling process.