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期刊论文

Synthesis of CCK-8 Tetrapeptide Fragment by Enzymatic Method

项光亚XIANG GuangyaHeiner Eckstein

华中科技大学学报[医学(英德文)版],2003,23(3):234~235,-0001,():

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摘要/描述

The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cho1ecystokinin C-terminal octapeptide CCK-8, was reported.This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-0Me and Met-OEt successively.These three steps were catalyzed by α-chymotrpsin. Papain and a-chymotrpsin respectively.The resuhs of FAB—MS showed that all the products had the correct molecular mass.

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