Genetically modified (GM) tomatoes have approved for commercialization in many countries since the first GM tomato FLAVER AVAR was permitted for planting in 1994. In 1994. In China, GM tomato Huafan No1 with a character of long shelf-life was the first GM plant which was approved forcommercialization in 1996. To meet the require ment of the GM tomatoes labeling policy that has been universal elements transformed into tomato, such as cauliflower mosaic virus 35S (CaMV35s) promoter and the nopaline synthase ●(NOS) terminator of Agrobacterium tumefaciens, and the specifically inserted heterologous DNA sequence between CaMV35s promoter and anti-sense ethylene-forming enzyme ●(EFE) gene were set up. To make the detection methods normative, a novel single copy tomato gene LAT52 was also used as an endogenous reference gene in the PCR detection systems. The limit of detection of screening and construct specific detection methods for Huafan No1 was 68 haploid genome copies in conventionl PCR detection, and three copies in TaqMan real-time PCR detection. The limit of quantitation of screening quantitative PCR assays for Huafan No1 was three copies and was 25 copies for construct-specific quantitative PCR. Two samples with know Huafan No1 was three copies and was 25 copies for construct-specific quantitative PCR. Two samples with known Huafan No1 tomato content were detected using the established conventional and real-time PCR systems, and these results also indicted that the established Huafan No1 screening and construct-specific PCR detection systems were reliable, sensitive and accurate.