The aim of this paper is to realize fully the hybrid respirometric measurement principle with high accuracy and measuring frequency. Based on the knowledge of the difficulty in implementing “ultimate” hybrid respirometer and the drawbacks of the simpler hybrid respirometer, a novel implementation of the hybrid respirometric measurement principle was developed. It was verified that the new hybrid respirometer possessed the advantage of “ultimate” hybrid respirometer while its DO electrodes suffered no effect of flow rate change. Also the accurate and constant temperature of the whole set-up was solved. For the purpose of enhancing the availability of the respirometer proposed in this paper, software with friendly user interface was developed, in which advanced arithmetic and median filter were adopted. Finally the new hybrid respirometer system was evaluated and the accuracy and reliability were satisfied.