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甘蓝型油菜(Brassica napus L.)矮秆突变位点的RAPD标记

赵云李华鹏周云涛王茂林*

四川大学学报(自然科学版),2004,41(6);1290~1293,-0001,():

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摘要/描述

50 10-mer RAPD primers were used to identify RAPD (random amplified polymorphie DNA) makers linked to the newly mutated dwarf locus in B. napus L. Between two DNA pools of the dwarf and its wild type parent, PCR results d 7 primers showed polymorphisms and all the specific DNA frdgment.s could only be amplified in high parent rather than in the mutant. In the F2 population of the hybrids of the wild type and the mutant, 4 of the 7 markers were identified to be linked to the mutated locus: 5910, S12610, S29390 and 47410, with a map distance of 16.6(eM), 0.0(eM), 16.6(eM) and 5.0(eM ) respectively. The potential uses d these molecular makers were also discussed.

【免责声明】以下全部内容由[赵云]上传于[2011年06月07日 08时55分35秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

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