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【期刊论文】转hTNF-α基因鱼腥藻7120的培养及外源基因的表达调控
郭勇, 刘志伟, 张晨
工业微生物2001年9月第31卷第3期/Industrial Microbiology Sept. 2001, Vol. 31, No. 3,-0001,():
-1年11月30日
讨论了转hTNF一口基因鱼腥藻7120外潭基因的调控和重组质舡的稳定性问题,并探索了鱼腥藻高效培养的方法和获得大量外源基因产精的途径。
鱼腥藻, 肿瘸坏死因子, 外源基因, 基因表达调控, 培养
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【期刊论文】高产黄酮苷银杏悬浮培养细胞系选育和继代培养稳定性研究
郭勇, 刘佳佳, 郑穗平, 张明浩
生物工程学报2001年1月/Chinese Journal of Biotechnology January 2001, Vol. 17, No. 1,-0001,():
-1年11月30日
Investigate the influence of culture media to growth and fhvonoI glycoside synthesis of calli introduced from seed ling of Cinkgo biloba.6 cell lines were selected from calll by hypoxla stress.Among these cell Lines the best one TZ1 which growth index was 4.12 and the flavonol glycoside content was 1.25% in dried cellwhichwss enhanced 257.1% compared with callus.The stability in subcultures wss investigated;The average content of flavonol glycoside wss 1.25% in dried cells and the growth index was 3.99 during 6 subeultures.Which variation coefficient was separately 0.065 and 0.048 The results show that hypoxia stress is a efficient method to select suspension cell line of higher prod uctivity of flavonol glycoside.
黄酮苷, 悬浮细胞系, 继代培养, 稳定性
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