用分步流延干燥法制备了一种由壳聚糖(CS) 、聚乙烯醇( PVA) 、羧甲基壳聚糖(CM-CS) 构成的新型人工皮肤医用材料——壳聚糖复合不对称膜(C-P-C 膜) ，其上层为CS 层，中间为PVA 层，下层为CM-CS 层。研究结果表明，可通过调节CS、PVA、CM-CS 的加入量来控制复合不对称膜各层的厚度和膜的水蒸气通透性， C-P-C 膜具有良好的透光性和吸水率。生物相容性实验发现，C-P-C 膜是无毒材料，不会引起创伤感染，随着植入C-P-C膜时间的延长，组织炎症反应明显减轻，6 周时，炎症浸润程度低于手术缝合线。C-P-C膜能够明显地促进创面愈合，能有效地密封出血创面且具有显著的止血作用。

利用生物化学和细胞培养方法对研制出的新型羧甲基壳多糖微载体CX-2 的生物亲和性、细胞贴壁及生长和扩展特性进行了研究。研究结果显示:CX-2 在pH4～7.2 范围内对小牛血清和人血清有较强的选择性吸附作用,表现出良好的生物亲和性;BB (Bulltrout Branchia) 细胞贴壁率、贴壁速率、生长速度和扩增培养效果表明:CX-2 微载体具有良好的细胞相容性,细胞能快速贴壁、生长和扩增;Vero 、BHK细胞在CX-2 微载体上贴壁、生长和扩增与BB 细胞基本一致,表明该微载体具有广泛的适应性。实验结果显示:CX-2 不仅具有优良的理化性能,同时具有良好的生物亲和性和细胞相容性,是一种应用前景广阔、实用性强的新型微载体。

In a new approach to the preparation of solid chitosan acetate, the dependence of solubility of chitsoan acetate on the mole ratio of acetic acid to GlcN residues of chitosan was evaluated from turbidity. The structure of the product chitosan acetate was characterized by titration and FT-IR. It was demonstrated that the chitosan acetate with high solubility retained the structure and antibacterial activity of chitosan. The antibacterial activities against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) have been exhibited by a special kind of agar and further investigated by optical density method. It was found that chitosan acetate at a concentration of 0.1% (m/V) exhibited some antibacterial activity but some of the bacteria did still grow. With a concentration of 0.15% (m/V), there were nearly no bacteria grown. Electron micrographs revealed bacterial action patterns of chitosan acetate towards E. coli and S. aureus.

A noval cellulose acetate/chitosan multimicrospheres (CACM) was prepared by the method of w/o/w emulsion. The concentration of cellulose acetate (CA) and the ratio of CA/chitosan (CS) had influence on the CACM size, and appearance. Ranitidine hydrochloride loading, and releasing efficiency in vitro were investigated. The optimal condition for preparation of the microspheres was CA concentration at 2% and the ratio of CA/CS at 3/1. The microspheres size was 200-350 μm. The appearance of microspheres was spherical, porous, and nonaggregated. The highest loading efficiency was 21%. The ranitidine release from the CACM was 40% during 48 hr in buffers.

Chitosan microcarriers (100-200μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120 ◦C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.