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黄鹰, Ying Huang, Mitsuhiro Hamada, and Richard J. Maraia‡
Vol. 275, No. 40, Issue of October 6, pp. 31480-31487, 2000,-0001,():
-1年11月30日
Eukaryotic tRNA genes are controlled by proximal and downstream elements that direct transcription by RNA polymerase (pol) III. Transcription factors (TFs) that reside near the initiation site are related in Saccharomyces cerevisiae and humans, while those that reside at or downstream of the B box share no recognizable sequence relatedness. Human TFIIICb is a transcriptional regulator that exhibits no homology to S. cerevisiae sequences on its own. We cloned an essential Schizosaccharomyces pombe gene that encodes a protein, Sfc6p, with homology to the S. cerevisiae TFIIIC subunit, TFC6p, that extends to human TFIIICb. We also isolated and cloned S. pombe homologs of three other TFIIIC subunits, Sfc3p, Sfc4p, and Sfc1p, the latter two of which are conserved from S. cerevisiae to humans, while the former shares homology with the S. cerevisiae B boxbinding homolog only. Sfc6p is a component of a sequence-specific DNA-binding complex that also contains the B box-binding homolog, Sfc3p. Immunoprecipitation of Sfc3p further revealed that Sfc1p, Sfc3p, Sfc4p, and Sfc6p are associated in vivo and that the isolated Sfc3p complex is active for pol III-mediated transcription of a S. pombe tRNA gene in vitro. These results establish a link between the downstream pol III TFs in yeast and humans.
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【期刊论文】Control of Transfer RNA Maturation by Phosphorylation of the Human La Antigen on Serine 366
黄鹰, Robert V. A. Intine, Amy L. Sakulich, Shashi B. Koduru, Ying Huang, Erik Pierstorff, John L. Goodier, Lon Phan, and Richard J. Maraia*
Molecular Cell, Vol. 6, 339-348, August, 2000, Copyright,-0001,():
-1年11月30日
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【期刊论文】Mechanism of Ribose 2¢-Group Discrimination by an RNA Polymerase†
黄鹰, Ying Huang, ‡ Fritz Eckstein, § Robert Padilla, ‡ and Rui Sousa*, ‡
Biochemistry 1997, 36, 8231-8242,-0001,():
-1年11月30日
The mechanism by which T7 RNA polymerase (RNAP) discriminates between rNTP and dNTP substrates has been characterized. During transcript elongation T7 RNAP uses rNTPs 70-80-fold more efficiently than dNTPs. Discrimination of the hydrogen-bonding character of the ribose 2¢-substituent contributes a largely Km-mediated factor of 20 to this preference for rNTPs. Discrimination of 2¢- substituent H-bonding character appears to be made through a hydrogen bond to the hydroxyl group of tyrosine 639. This hydrogen bond makes little net contribution to either rNTP ground or transition state binding energy apparently because it is balanced by the energy of desolvation of the tyrosine hydroxyl. This mechanism may reflect a strategy to facilitate translocation by minimizing contributions from polymerase-NMP moiety interactions to NTP binding energy so as to minimize the affinity of the NTP binding site for the 3¢-NMP of the product nucleic acid.
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黄鹰, Ying HUANG, Preston N. GARRISON and Larry D. BARNES*
Biochem. J. (1995) 312, 925-932 (Printed in Great Britain),-0001,():
-1年11月30日
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