以东海原甲藻Prorocentrum donghaiense Lu为试验材料,设置了短光周期（A）和长光周期（B）两组试验：A组分光周期1、2、4、6、8、10和12 h 7个处理,B组分光周期12、14、16、18、20、22和23 h 7个处理,测定了东海原甲藻的细胞密度、叶绿素a含量、蛋白质含量、生物量和可溶性糖等指标。结果表明,在13个不同的光周期处理中,随着光周期的不断延长,东海原甲藻的细胞密度等一系列指标值均不断上升,各指标和光周期的长度呈现正相关的关系。结果表明东海原甲藻是一种喜长光照的赤潮藻类。

The zooplankton of 18 reservoirs of South China was investigated in 2000. 61 Rotifera species, 23 Cladoceras and 14 Copepodas were identified. The most frequent Rotifera genera were Keratella, Brachionus, Trichocerca, Diurella, Ascomorpha, Polyarthra, Ploesoma, Asplanchna, Pompholyx and Conochilus. Bosmina longirostris, Bosminopsis deitersi, Diaphanosoma birgei, D. brachyurum and Moina micrura were typical of Cladocera in the reservoirs. Phyllodiaptomus tunguidus, Neodiaptomus schmackeri and Mesocyclops leuckarti were the most frequent Copepoda and M. leuckarti dominated Copepoda in most reservoirs. High zooplankton species richness with low abundance was characteristic of the throughflowing reservoir, whereas low species richness with low abundance was found in the reservoir with the longest retention time. Relative high abundance and medium species diversity were the distinction of intermediate retention time reservoirs.

通过对眼点拟微绿球藻和扁藻进行共培养试验,结果表明,在共培养条件下,眼点拟微绿球藻和扁藻的生长速度均受到抑制,即两种藻的最大生长密度均比对照（分别单独培养）有显著降低,而且眼点拟微绿球藻降低的程度要大于扁藻。经计算两种藻的种间竞争系数可知,扁藻的种问竞争能力明显强于眼点拟微绿球藻。

采用LRH -GII型光照培养箱培养试验藻种,设置了持续黑暗胁迫（分6个强度处理）和间断黑暗胁迫（分4个模式处理）两项试验,研究了眼点拟微绿球藻(Nannochloropsis oculata)在不同环境胁迫下的生理生态变化和该藻种在黑暗胁迫下的超补偿生长响应情况．两个试验的结果均显示,眼点拟微绿球藻在持续黑暗胁迫和间断胁迫后,均表现出明显的超补偿作用现象．黑暗对眼点拟微绿球藻的生长造成显著抑制,但是适当进行黑暗胁迫以后恢复生长,微藻具有更强的生长繁殖能力,细胞净增率最大可达123%．经SSR测验,不同的胁迫方式、胁迫持续时间和胁迫模式与超补偿现象发生时间及强度密切相关,

The single-chain insulin (PIP) can spontaneously fold into native structure through preferred kinetic inter-mediates. During refolding, pairing of the first disulfide A20–B19 is highly specific, whereas pairing of the second disulfide is likely random because two two-disulfide intermediates have been trapped. To get more details of pairing property of the second disulfide, four model peptides of possible folding intermediates with two disulfides were prepared by protein engineering, and their properties were analyzed. The four model peptides were named [A20–B19, A7–B7]PIP, [A20–B19, A6–B7]PIP, [A20–B19, A6–A11]PIP, and [A20– B19, A7–A11]PIP according to their remaining disulfides. The four model peptides all adopt partially folded structure with moderate conformational differences. In redox buffer, the disulfides of the model peptides are more easily reduced than those of the wild-type PIP. During in vitro refolding, the reduced model peptides share similar relative folding rates but different folding yields: The refolding efficiency of the reduced [A20–B19, A7–A11]PIP is about threefold lower than that of the other three peptides. The present results indicate that the folding intermediates corresponding to the present model peptides all adopt partially folded conformation, and can be formed during PIP refolding, but the chance of forming the intermediate with disulfide [A20–B19, A7–A11] is much lower than that of forming the other three intermediates.