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毕开顺, Kang LI, Xiaohui CHEN, Ying JIA, and Kaishun BI
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-1年11月30日
A sensitive, simple, and accurate method for the determination and pharmacokinetic study of vanillic acid in rat plasma was developed using reverse-phase HPLC with UV detection after oral administration of the traditional Chinese medicine preparation of the Di-Gu-Pi decoction. Plasma samples taken from rats were extracted with methanol. The constituent vanillin acid was separated on a C18 stationary phase and a mobile phase of acetonitrile-water (15:85, v/v) (adjusted to pH 3.0 using phosphoric acid), with a UV detector setting at 260 nm. The validated HPLC method developed was used to determine the pharmacokinetic profile of vanillin acid in rat plasma after administration of the Di-Gu-Pi decoction.
Di-Gu-Pi decoction, vanillic acid, pharmacokinetics
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毕开顺, Q.-F. Liao, Y. Jia, Q.-T. Gao, X.-H. Chen, X.-J. Tan, K.-S. Bi
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-1年11月30日
A simple and sensitive high-performance liquid chromatographic method has been developed for determination of chlorogenic acid in rat plasma. Chlorogenic acid was extracted from plasma samples with methanol. HPLC analysis of the extracts was performed on a C18 column (250 mm × 4.6 mm i.d.5 µm particles). The mobile phase was acetonitrile -1% formic acid (9:91, v/v). The calibration plot was linear over the range 0.0420-2.10 µg mL-1 and the lower limit of quantification was 0.0420 μg mL-1. The method was reproducible and reliable with intra-day pre-cision better than 8.2%, inter-day precision better than 9.1 %, accuracy within ±8.3%, and mean extraction recovery above 84.4%. The validated method was successfully applied to pharmacokinetic studies of photogenic acid in rat plasma after administration of Luying decoction.
Column liquid chromatography, Chlorogenic acid, Pharmacokinetic studies, Luying decoction, Herba sambuci chinesis
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毕开顺
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-1年11月30日
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【期刊论文】Analysis of the essential oil from radix bupleuri using capillary gas chromatography
毕开顺
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-1年11月30日
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【期刊论文】Analysis of the fatty acid from Bupleurum Chinense DC in China by GC-MS and GC-FID
毕开顺
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-1年11月30日
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