Lactferrin (LF)是一种铁结台性糖蛋口质，最初作为抗菌蛋白质被广泛研究。 LF在多种哺乳类乳汁中广泛含有，但在人乳中含量最高。 LF能够争夺微生物生长所需铁离子，并且能激活自然杀伤（NK）细胞和中性粒细胞，诱导集落刺激活性，刺激淋巴因子激活杀伤（LAK)细胞，增强巨噬细胞的细胞毒作用，调整肠内环境改善肠蠕动，预防 c型肝炎病毒的感染，预防癌发生及癌转移，本文对此作了综述。

目的：探讨中药复方肠安泰对肠管绒毛黏膜固有层B细胞及 IL-l2的影响及其与肺转移的关系。方法：经皮下植入大肠癌高度肺转移细胞(Co26Lu)，建立 大肠癌肺转移小鼠动物模型 ，采用 ABC免疫染色及免疫荧光染色方法做相关分析。结果：荷瘤治疗组与荷瘤对照组相比 IgA、IgM及IL-12阳性细胞在小肠绒毛黏膜固有层均有显著意义的增加。两组数值依次为 172.3±6.7，59.2±5.3，79.6±4.1；131.2±5.3，45.1± 4.3，24.4± 2.5(P<0.01)。结论：中药复方肠安泰预防大肠癌肺转移的机制，与其促进了荷瘤小鼠小肠绒毛黏膜 固有层B细胞的活化及 IL-12的诱导有关。

We have previously demonstrated that oral administration of bovine lactoferrin (bLF) markedly increases CD4+ and CD8+ T cells and NK (asialoGM1+) cells in the blood of tumor-bearing mice and enhances anti-metastatic activity. In this paper, we document that oral administration of bLF and bLF-hydrolysate (bLFH) is associated with strong increases in CD4+ and CD8+ T, as well as asialoGM1+ cells in lymphoid tissues and lamina propria of the small intestine in mice, especially in tumor-bearing animals in which Co26Lu cells were implanted subcutaneously. Moreover, IgM+ and IgA+ B cells in lamina propria of the small intestine were also significantly increased by bLF and bLFH. Bovine apo-transferrin (bTF) did not exhibit such activity. In the colon, only CD8+ cells were significantly increased by treatment with bLF, while asialoGM1+ cells were significantly decreased. bLF and bLFH induced cytokines to activate T, B and asialoGM1+ cells. Administration of bLF and bLFH, but not bTF, increased production of interleukin-18 (IL-18), interferon-gamma (IFN-γ ) and caspase-1 in the mucosa of the small intestine. Particularly high levels of IL-18 were found in the epithelial cells of the small intestine. Moreover, administration of bLF and bLFH, but not bTF, induced IFN-γ presenting cells in the small intestine. Caspase-1, which processes proIL-18 to mature IL-18, was also induced in the epithelial cells of the small intestine following treatment with bLF and bLFH, but not with bTF. These results suggest that enhanced production of IL-18 and IFN-γ and caspase-1 induction by treatment with bLF may be important for elevation of intestinal mucosal immunity.

目的：应用血清药理学方法检测中药肠安泰及其主要成分的药物血清对 Co26lu大肠癌细胞体外杀伤能力。方法：5组大鼠除 5-Fu血清组按30mg/kg予腹腔注射，给药 2h后腹主动脉采血外，其余4组均 按0.6ml·kg-1·d -1分别给予肠安泰 (干膏)、大黄 (干膏)、藤梨根 (干膏)、生理盐水灌胃，选用2种 不同采血时间。一 日采血：用药 1h后眶静脉采血 2ml；三 日采血：末次用药后 1h腹主动脉采血，无菌分离血清。采用 MTF法观察药物血清对细胞的杀伤作用。结果：肠安泰及其单味药与 5-Fu的不同浓度药物 血清对Co26lu细胞均有不同程度的体外杀伤能力，并呈剂量依赖性。结论：肠安泰能够抑制肿瘤细胞的生长及转移。